Fig. 1
From: CXCR4-targeted nanotoxins induce GSDME-dependent pyroptosis in head and neck squamous cell carcinoma
T22-PE24-H6 and T22-DITOX-H6 CXCR4-dependent cytotoxic effect in HNSCC cell lines. A and B) T22-PE24-H6 (A) and T22-DITOX-H6 (B) cytotoxic effect (0-50 nM) after 48 h of exposure in 22A mock and 22A-CXCR4+ cell lines represented as percentage of cell viability and IC50 values. C and D) T22-PE24-H6 (C) and T22-DITOX-H6 (D) cytotoxic effect (0–50 nM) after 48 h of exposure in 74B mock and 74B-CXCR4+ cell lines represented as percentage of cell viability and IC50 values. E and F) AMD3100 blocking assay (1 μM) in 22A-CXCR4+ and 74B-CXCR4+ cell lines treated with T22-PE24-H6 (E) (5 nM for 22A-CXCR4+ and 50 nM for 74B-CXCR4+) and T22-DITOX-H6 (F) (50 nM) for 48 h. ** p < 0.01; *** p < 0.001. Each column represents the mean value of three biological replicates. Statistical analysis performed by Student t-test. Error bars indicate SEM