Fig. 5
From: CXCR4-targeted nanotoxins induce GSDME-dependent pyroptosis in head and neck squamous cell carcinoma
Cytotoxic effect of T22-PE24-H6 and T22-DITOX-H6 in tumor tissue. A Detection of dead cells by condensate DNA staining (DAPI) in the buffer, T22-PE24-H6 or T22-DITOX-H6 treated. Quantification of the number of DAPI positive stained cells in tumor tissue represented as fold-change respect to the buffer. B IHC analysis of tumor infiltrated macrophages in tumors from buffer, T22-PE24-H6 or T22-DITOX-H6 treated animals detected by F4/80 immunostaining. Quantification of the percentage of F4/80 positive stained cells in tumor samples from each group. Scale bars = 50 μm. F4/80 expression was quantified as mean gray value and represented as mean ± SEM. ** p < 0.01; *** p < 0.001. Statistical analysis performed by Student t-test