Fig. 4
From: Partial p53 reactivation is sufficient to induce cancer regression
In vivo response of p53-deficient AML to E177R activation. a Mice transplanted with LSL AML cells were sacrificed before and day 1, 3, 7 and 11 after start of 1 week of daily treatment with Tam or corn oil (mock). Samples were analyzed by immunohistochemistry for p53E177R, GFP (as a marker of AML cells), BrdU (proliferation), senescence-associated β-galactosidase (SA β-gal), apoptosis (TUNEL) and CD3 as a marker of immune infiltration. Arrowheads indicate senescent cells. b-d Quantification of immunohistochemistry analysis. b Senescence-associated β-galactosidase. c Apoptosis (TUNEL). d Immune infiltrate (CD3). All bar graphs show the mean positivity index ±SD; datapoints represent individual fields of view (1000X1000 pixel each) from n = 2 mice sacrificed at each time point; reported are P-values of 1way ANOVA and indicated pairwise comparisons (Dunnett’s multiple comparisons test)