Skip to main content
Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Partial p53 reactivation is sufficient to induce cancer regression

Fig. 6

E177R activation induces regression of thymic lymphomas in p53-deficient mice. Trp53LSL-E177R/LSL-E177R mice (p53LSL/LSL) with the Rosa26CreERT2 (CreERT2) transgene (n = 17) were treated 1 week with daily i.p. injections of 1 mg tamoxifen at 4 months of age and euthanized when humane endpoint criteria were reached. p53LSL/LSL mice (without CreERT2) treated with tamoxifen served as controls (n = 22). a Kaplan-Meier survival graph with Log-rank (Mantel-Cox) test. b-d Individual mice (p53LSL/LSL; CreERT2 (red): n = 9; p53LSL/LSL (blue): n = 8) that were diagnosed with thymic lymphoma by MRI received tamoxifen treatment and were re-examined by MRI 7, 14, and 28 days after treatment start. b Shown is the fold-change in tumor volume (determined by MRI) relative to pre-treatment for individual animals. Insert shows the mean absolute tumor volume ± SD in both cohorts at the start of treatment; data points represent individual mice; two-tailed unpaired t-test. c Shown is the maximum response (fold increase in tumor volume) in individual mice. Reported is the P-value of a two-tailed Mann-Whitney test comparing tamoxifen treatment responses of p53LSL/LSL; CreERT2 (red) and p53LSL/LSL (blue) mice. d Representative MRI results from one mouse of each cohort. The p53LSL/LSL mouse without CreERT2 (blue) progressed under treatment and reached the endpoint before day 14. The p53LSL/LSL mouse with CreERT2 (red) showed tumor regression until day 14 and presented with relapse on day 28. Shown are tumor sections in all 3 dimensions; tumors are highlighted in color. e-f Cancer types detected in both cohorts after treatment when the animals reached the humane endpoint criteria. e Fraction of thymic lymphomas versus non-thymic tumor types (including mostly sarcomas and splenic B cell lymphomas). f Representative histological images of non-regressed undifferentiated sarcomas in n = 6 different animals

Back to article page