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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Brucein D augments the chemosensitivity of gemcitabine in pancreatic cancer via inhibiting the Nrf2 pathway

Fig. 2

BD enhances the sensitivity of GEM in human PDAC cells. a The chemical structure of BD. b Miapaca-2, Capan-2 and PANC-1 cells were treated with different concentrations of BD for 48 or 72 h, and the cell viability was assessed using MTT assay. c Pancreatic cell lines treated with BD (0.5 µM) or/and GEM (50 µg/mL) for 48 h, and MTT assay was used to measure the cell viability. d-e Colony formation assay. f-g Flow cytometric analysis for cellular apoptosis. h The expression level of Caspase-3, caspase-9 and PARP subjected to BD and GEM treatment in Miapaca-2, Capan-2 and PANC-1 cells were detected by western blot. i Flow cytometry analysis of ROS levels in PDAC cells treated with BD or GEM and their combination. Data were presented as the mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with the control group. #p < 0.05, ##p < 0.01 compared with the GEM alone treatment group

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