Fig. 7

BD promotes the ubiquitin–proteasome dependent degradation of Nrf2. a The relative Nrf2 mRNA levels as analyzed by qRT-PCR. b-c Miapaca-2 and Capan-2 cells were treated with 25 μM CHX with or without BD (1.5 μM) for the indicated time and the cell lysates were analyzed by immunoblotting. d Miapaca-2 and Capan-2 cells treated with BD (1.5 μM) for the indicated time periods (0–48 h), and the level of ubiquitin was detected by immunoblotting using an anti-ubiquitin antibody. e–f Miapaca-2 and Capan-2 cells were treated with BD (1.5 µM), MG132 (20 µM), or the combination of BD and MG132 for 8 h, and the protein level of Nrf2 were detected by immunoblotting. Data were presented as the mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with the control group