Fig. 6

EMILIN-2 loss tilts the macrophage polarization towards the M2 phenotype. A qPCR analysis of the expression of the M1 markers NOS-2 and IL-1β, relative to that of GAPDH, in bone marrow derived macrophages from wild type (wt) and Emilin-2^−/− (E2^−/−) mice upon IFNγ/TNF-α treatment in the presence of recombinant EMILIN-2 (rE2) or vehicle (PBS); n = 4. B qPCR analysis of the expression M2 markers Chil3 and Arg-1, relative to that of GAPDH, in bone marrow derived macrophages from wild type (wt) and Emilin-2^−/− (E2^−/−) mice upon IL-4/IL-10 treatment in the presence of recombinant EMILIN-2 (rE2) or vehicle (PBS); n = 4. C Western blot analyses of the expression of NOS-2 and Arg-1 in bone marrow-derived macrophages from wild type (wt) and Emilin-2^−/− (E2^−/−) mice upon treatments with IFNγ/TNF-α or IL-4/IL-10 in the presence of recombinant EMILIN-2 (rE2) or vehicle (PBS); n = 3. D and E Quantification of the results reported in C. Graphs represent the mean ± SD; P values were obtained using the One Way Anova Test; * P < 0.05, **P < 0.01, ***P < 0.001, n.s.: P > 0.05