Fig. 4

A Mechanism of ZGd-NR-sensitized radiation for ICD induction and TAM depletion to synergistically boost antitumor immunity. B-K Immunogenic cell death induction and immunosuppressive tumor microenvironment reprogramming. B Immunofluorescence of CT26 cells stained with anti-CRT antibody, scale bar = 20 μm. C Quantification of relative CRT mean fluorescence intensity (n = 5 biologically independent cells). D Detection of cytoplasmic HMGB1 by ELISA kit (n = 5 biologically independent cells). E Detection of ATP secretion by luciferin-based ATP assay kit (n = 5 biologically independent cells). F Regulation of tumor immunity by TAMs. G Flow cytometry analysis of TAMs (F4/80+ and CD11b+) in tumor tissues (n = 10 biologically independent animals). H Quantification of the levels of TGF-β1 (I), IL-10 (J), and VEGF-A in tumor tissues by ELISA kit, the tumor tissues were harvested 48 h after radiotherapy (0 or 6 Gy × 1, n = 8 biologically independent animals). K Flow cytometry analysis of DCs maturation (CD80+ and CD86+ gated on CD11c+) in tumor-draining lymph nodes; the TDLNs were harvested 5 days after radiotherapy (0 or 6 Gy × 1, n = 8). All data were shown as mean ± SD. N.S. represented non-significance, *p < 0.05, **p < 0.01, ***p < 0.001 [141]