Fig. 1

CircHNRNPU is the most abundantly and differentially expressed circRNA in IgD MM. A Agilent SBC-ceRNA microarray chips was employed to detect circRNA expression in 3 normal plasma cell samples (NPCs), 5 lgD MM samples and 5 lgG MM samples respectively. B The volcano plot of differentially expressed circRNAs between IgD and IgG MM tissues (n = 5). X axis, −log10 P value; Y aixs, log2 fold change. C Seven modules were enriched in the differentially expressed circRNA and mRNA in IgD MM using Combined weighted gene correlation network analysis (WGCNA) (FPKM> 1 and at least expressed in 3 samples). D Turquoise and green modules were the genes that highly expressed in IgD MM patients compared with IgG MM samples and their paired normal tissues. E-F CircHNRNPU (hsa_circ_0017272) was the most abundantly and differentially expressed circRNA in IgD MM compared with IgG MM and their paired normal tissues. G HNRNPU mRNA levels were significantly increased in MM samples. The signal level of HNRNPU was shown on the y-axis. Patients were designated as healthy donors with normal bone marrow plasma cells (NP, n = 22), monoclonal gammopathy of undetermined significance (MGUS, n = 44), or multiple myeloma (MM, n = 351), sorted on the x-axis. H-J Increased HNRNPU mRNA expression was associated with poor overall survival (OS) in MM patients from (H) TT2, (I) GSE136337 and (J) HOVON65 patient cohorts. K In paired MM samples collected at first diagnosis and relapse, HNRNPU mRNA expression was increased in the relapsed samples relative to the first diagnosis samples. L-M Elevated HNRNPU expression was correlated with decreased OS in relapsed patients in (L) APEX and (M) TT2 cohorts