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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing

Fig. 3

CircHNRNPU encodes a novel HNRNPU isoform. A The putative open reading frame (ORF) in circHNRNPU. The sequences of the putative ORF were shown in green, internal ribosomal entrance site (IRES) sequences were shown in purple. B The predicted sequence of circHNRNPU_603aa. C WB analysis of endogenous circHNRNPU_603aa expression in HEK-293, ARP1 and CAG cells. D The specific peptides from circHNRNPU_603aa were identified by mass spectrometry analysis. E Illustration of endo-circHNRNPU and circHNRNPU-HA. F-G RNA levels of circHNRNPU and linear HNRNPU were determined by RT-PCR and qRT-PCR. Sanger sequencing following PCR was conducted using the indicated divergent primers to confirm the precise splicing of circHNRNPU-OE plasmid in ARP1 and CAG cells. The hsa_circ_0000284 was used as internal control gene. H WB analysis of circHNRNPU_603aa overexpression in HEK-293, ARP1 and CAG cells detected by HNRNPU and HA tag antibody. I The specific peptides from circHNRNPU_603aa were identified by mass spectrometry analysis. J Confocal microscope live image was used to capture circHNRNPU_603aa cellular localization. Scale bars, 25 μm. The data are presented as mean ± SD.*p < 0.05, **p < 0.01, ***p < 0.001

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