Fig. 5

Identification of circHNRNPU_603aa-regulated AS events. A Number of AS events in each category. B GO Molecular Function enrichment of circHNRNPU_603aa-regulated AS genes. C The pathway enrichment analysis of above genes revealed that their molecular function was centered on ubiquitin mediated proteolysis. D The expression of SKP2 exon 5 skipping splice variants was significantly increased by increased circHNRNPU_603aa and ranked top of the circHNRNPU_603aa-regulated AS events. E-G Survival proportions of patients corresponding to different probes of SKP2 in APEX cohort. 203626_s_at was related to better survival compared with other probes. H-J The expression of SKP2-NM_001243120.2 in circHNRNPU_603aa-OE and si-circHNRNPU_603aa cells relative to control cells. A two-tailed Student’s t-test was utilized to evaluate statistical significance. K-L Overexpression of circHNRNPU-603aa directly upregulated SKP2-NM_001243120.2 and downregulated SKP2-NM_005983.4 detected by RIP-qPCR. The data are presented as mean ± SD.*p < 0.05, **p < 0.01, ***p < 0.001