Fig. 4
LINC00930 interacts with RBBP5 and GCN5. a The LINC00930-interacting proteins were annotated with their Log10 ratio in 5-8F and CNE2 cell lysate. b Western blot of the proteins from antisense LINC00930 and LINC00930 pull-down assays. c RNA immunoprecipitation experiments were performed using anti-RBBP5 or anti-GCN5 antibody, and specific primers were used to detect LINC00930 or GAPDH. d Nuclear lysates of 5-8F were immunoprecipitated with anti-RBBP5 antibodies (Left), anti-GCN5 antibody (Right), or control IgG. Aliquots of nuclear lysates (20% of input) and the RBBP5 or GCN5 immunoprecipitates were separated by SDS-PAGE, and the specific immunoprecipitation of RBBP5 and GCN5 was confirmed by western blot (WB). The complexes were analyzed for the presence of LINC00930 or GAPDH by real-time PCR. e Co-IP assay detected the interaction of RBBP5 and GCN5 in the 5-8F cell. The 20% of input (cell lysate) and RBBP5 or GCN5 immunoprecipitates were separated by SDS-PAGE. The specific immunoprecipitation of RBBP5 and GCN5 was confirmed by Western blot. f IP assay was performed to detect the interaction between RBBP5 and GCN5 after LINC00930 knockdown. g Western blot of RBBP5 and GCN5 in samples pulled down by full-length (FL) or truncated LINC00930 (Δ1: 1–450, Δ2: 451–900, Δ3: 901–1350, Δ4: 1351–1762). h Top: Mutation on W279, K281 and G284 of RBBP5 impaired the RBBP5-LINC00930 association. Bottom: Mutation on N291, R294 and C297 of GCN5 impaired the GCN5-LINC00930 association