Fig. 4

RBPJ specifically binds the THY1 gene promoter. A In-silico prediction of RPBJ binding sites on the THY1 gene promoter using the EPD new software, setting a P-value cut-off of 0.01. Binding sites are represented in red squares. B The Notch pathway was inhibited by transient overexpression of a dominant-negative form of the Notch transcriptional co-activator RBPJ (dnRBPJ). After transfection, THY1 gene expression levels were significantly reduced (***p < 0.001), showing that THY1 expression is downstream of the Notch1 pathway. C Chromatin Immunoprecipitation (ChIP) in KKUM-213, KKUM-156, and RBE cells. Chromatin was pulled down with anti-RBPJ (#1 and #2), -H3K4me3, and -H3K27me3 antibodies. Anti-IgGs were used as a negative control