Fig. 5

Functional study of KKU-M213 in vitro. A-B THY mRNA and CD90 protein levels were downregulated by 60% and 40% respectively, in silenced cells, as compared to sh-RNA cells.. C Representative spheroids images at 6–10-14 days of KKU-M213_scramble shRNA compared to KKU-M213_THY1/CD90-shRNA cells. Downregulation of THY1 expression significantly reduces spheroids of KKU-M213 cells. Spheroids diameter (μm) was measured and values reported in the graph below represent the mean ± SEM of three independent experiments relative to the control. **p < 0.01 calculated with Student’s t-test. (Magnification: 10 x)