Fig. 6

Functional study of HuCCT1 in vitro. A-B A 275-fold increase of mRNA expression and a threefold increase in protein levels are detected in HuCCT1 THY1/CD90 +  + compared to scrambled control. C Representative sphere-forming capacity images of HuCCT1_CD90 +  + compared to HuCCT1_empty vector at 6–10-14 days. Increased THY1 expression induces a greater number of tumor colonies and with a larger diameter, of HuCCT1 THY1/CD90 +  + cells. The diameter (μm) of the sphere was measured per microscopic field (Magnification: 10 x), and the values reported in the graph below represent the mean ± SEM of three independent experiments relative to the control. **p < 0.01; ***p < 0.001 calculated with Student’s t-test