Fig. 5

DICER1-AS1 promotes DICER1 transcription by recruiting YY1 to the promoter of DICER1. A Prediction of the consensus YY1 binding sequence was presented via the JASPAR database (upper panel). Schematic illustration showing the prediction of YY1 binding sites of DICER1 promoter via the JASPAR database (lower panel). B ChIP-Seq tracks from the Cistrome Data Browser database (http://cistrome.org/db/#/) showing the enrichments of H3K4me3, H3K27ac, and YY1 across the DICER1 promoter sequence. C Relative enrichment of YY1 (left panel) to DICER1 promoter was detected via ChIP assays using anti-YY1 antibody in BxPC-3 cells. The PCR products were run on a 2% agarose gel (right panel). D Schematic illustration showing the different mutants of YY1 binding sites of DICER1 promoter. E The luciferase reporter assays showing the relative activity of YY1 on DICER1 promoter in BxPC-3 cells using wild type (WT) or mutant-type (MUT)-containing promoter reporter vector. F Real-time qRT-PCR (upper panel) and western blot (upper panel) showing the changes of mRNA and protein levels of DICER1 in BxPC-3 and PANC-1 cells transfected with vector control, YY1, siNC, or siYY1 (#1, #2). G The luciferase reporter assays showing the relative activity of DICER1 promoter in BxPC-3 cells containing WT or MUT reporter vector. These cells were further transfected with vector, DICER1-AS1 and those co-transfected with siNC or siYY1. H Relative enrichment of YY1 to DICER1 promoter was detected via ChIP assays in PC cells transfected with empty vector, DICER1-AS1, and those co-transfected with siNC or siYY1. I Real-time qRT-PCR (left panel) and western blot (right panel) showing the changes of transcript and protein levels of DICER1 in BxPC-3 and PANC-1 cells transfected with vector or DICER1-AS1 plasmids and those co-transfected with siNC or siYY1. J-K The relative glucose uptake and relative lactic acid production were detected in PC cells transfected with empty vector or DICER1-AS1, and those co-transfected with siNC or siYY1. All data were presented as means ± SD of at least three independent experiments. Values are significant at ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001 as indicated