Fig. 4

ITGA2 silencing induced the expression of SMAD2 in pancreatic cancer cells. a-c RT-PCR was used to determine the mRNA expression level of SMAD2 (a), SMAD3 (b), and SMAD4 (c) in the PANC-1 and AsPC-1 cells infected with sh-Control or sh-ITGA2s. GAPDH served as an internal reference and repeated in triplicates. ns, not significant; ***P < 0.001. d Western blot analysis was used to determine the protein expression level of SMAD2, SMAD3, SMAD4, and p-SMAD2/3 in the PANC-1 and AsPC-1 cells infected with sh-Control or sh-ITGA2s. GAPDH served as an internal reference. e-g RT-PCR was used to determine the mRNA expression level of SMAD2 (e), SMAD3 (f) and SMAD4 (g) in PANC-1 and AsPC-1 cells infected with or without ITGA2 plasmids. GAPDH served as an internal reference and repeated in triplicates. ***P < 0.001. h Western blot analysis was used to determine the protein expression level of SMAD2, SMAD3, SMAD4, and p-SMAD2/3 in the PANC-1 and AsPC-1 cells infected with or without ITGA2 plasmids. GAPDH served as an internal reference