Skip to main content
Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Overexpressed integrin alpha 2 inhibits the activation of the transforming growth factor β pathway in pancreatic cancer via the TFCP2-SMAD2 axis

Fig. 5

ITGA2 inhibited the activation of TGF- β pathway via SMAD2 in pancreatic cancer cells. a-c RT-PCR was used to determine the mRNA expression level of CDKN2B (a), CDKN1A (b) and SERPINE3 (c) in the PANC-1 and AsPC-1 cells infected with sh-ITGA2 and/or sh-SMAD2. GAPDH served as an internal reference and repeated in triplicates. ns, not significant; ***P < 0.001. d Western blot analysis was used to determine the protein expression level of CDKN1A and CDKN2B in the PANC-1 and AsPC-1 cells infected with sh-ITGA2 and/or sh-SMAD2. GAPDH served as an internal reference and repeated in triplicates. ns, not significant; ***P < 0.001. e Western blot analysis was used to determine the protein expression level of CDKN1A and CDKN2B in the PANC-1 and AsPC-1 cells infected with ITGA2 plasmids and/or SMAD2 plasmids. GAPDH served as an internal reference and repeated in triplicates. ns, not significant; ***P < 0.001. f-h RT-PCR was used to determine the mRNA expression level of CDKN2B (a), CDKN1A (b) and SERPINE3 (c) in the PANC-1 and AsPC-1 cells infected with ITGA2 plasmids and/or SMAD2 plasmids. GAPDH served as an internal reference and repeated in triplicates. ns, not significant; ***P < 0.001

Back to article page