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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Overexpressed integrin alpha 2 inhibits the activation of the transforming growth factor β pathway in pancreatic cancer via the TFCP2-SMAD2 axis

Fig. 6

ITGA2 inhibited the expression of SMAD2 by interacting with TFCP2 in pancreatic cancer cells. a-b LC-MS/MS was used to identify the interaction of TFCP2 with ITGA2 (a) by detecting the peptided of TFCP2 (b). c Immunoprecipitation assay, showing the interaction between ITGA2 and TFCP2 in the PANC-1 and AsPC-1 cells. d RT-PCR was used to determine the mRNA expression level of SMAD2 in the PANC-1 and AsPC-1 cells infected with sh-ITGA2 and/or sh-TFCP2. GAPDH served as an internal reference and repeated in triplicates. ns, not significant; ***P < 0.001. e Western blot analysis was used to determine the protein expression level of SMAD2 in the PANC-1 and AsPC-1 cells infected with sh-ITGA2 and/or sh-TFCP2. GAPDH served as an internal reference. f RT-PCR was used to determine the mRNA expression level of SMAD2 in PANC-1 and AsPC-1 cells infected with ITGA2 plasmids and/or TFCP2 plasmids. GAPDH served as an internal reference and repeated in triplicates. ns, not significant; ***P < 0.001. g Western blot analysis was used to determine the protein expression level of SMAD2 in the PANC-1 and AsPC-1 cells infected with ITGA2 plasmids and/or TFCP2 plasmids. GAPDH served as an internal reference

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