Skip to main content
Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Overexpressed integrin alpha 2 inhibits the activation of the transforming growth factor β pathway in pancreatic cancer via the TFCP2-SMAD2 axis

Fig. 7

TFCP2 induced the expression of SMAD2 by acting as a transcription factor in pancreatic cancer cells. a GEPIA database was searched to determine the correlation between the mRNA expression levels of SMAD2 and TFCP2 in the PDAC samples. P- and R-values are indicated in the figure. b RT-PCR was used to determine the mRNA expression level of SMAD2 in PANC-1 and AsPC-1 cells infected with sh-Control or sh-TFCP2s. GAPDH served as an internal reference and repeated in triplicates. ns, not significant; ***P < 0.001. c Western blot analysis was used to determine the protein expression level of SMAD2 in the PANC-1 and AsPC-1 cells infected with sh-Control or sh-TFCP2s. GAPDH served as an internal reference. d RT-PCR was used to determine the mRNA expression level of SMAD2 in the PANC-1 and AsPC-1 cells infected with pcDNA3.1 or TFCP2 plasmids. GAPDH served as an internal reference and repeated in triplicates. ns, not significant; ***P < 0.001. e Western blot analysis was used to determine the protein expression level of SMAD2 in the PANC-1 and AsPC-1 cells infected with pcDNA3.1 or TFCP2 plasmids. GAPDH served as an internal reference. f EPD database was searched to determine the potential binding sites of TFCP2 in the promoter region in SMAD2 gene. g ChIP-qPCR of TFCP2 in the PANC-1 and AsPC-1 cells. All the data are shown as the mean values ± SD from three independent replicates. ***P < 0.001. h and i Western blot analysis was used to detect the protein expression level of TFCP2 in the cytoplasm and nucleus of PANC-1 cells and AsPC-1 cells infected with sh-Control or sh-ITGA2 (h) and pcDNA3.1 or ITGA2 plasmids (i). j Pancreatic cancer cells were divided into two groups, including treatment (sh-ITGA2) and control (sh-control) groups. The tissue sections were incubated with rabbit anti-ITGA2 antibody and rabbit anti-TFCP2 antibody for immunofluorescent to find the nuclear translocation of TFCP2

Back to article page