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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Targeting the radiation-induced ARv7-mediated circNHS/miR-512-5p/XRCC5 signaling with Quercetin increases prostate cancer radiosensitivity

Fig. 3

A ChIP assay of the two potential ARE binding sites on NHS promoter. B Co-transfection of wild-type ARE or mutant NHS promoter pGL3-Luciferase constructs into 22Rv1 cells with/without shARv7. Luciferase activity in 22Rv1 cells carried wild-type or mutant NHS promoter plasmids. C shcircNHS effects on C4-2-IRR cells survival after IR using clonogenic assay. D The oecircNHS effects on C4-2 cells survival after IR using clonogenic assay. E WB analysis of γ-H2AX in different groups. The oecircNHS can decrease DNA damage repair time in C4-2 cells and shcircNHS can increase DNA damage repair time in C4-2-IRR cells. F shcircNHS effects on DNA damage in C4-2-IRR cells after IR using comet assay. Scale bar = 10 µm. G The oeARv7 and/or shcircNHS effects on C4-2 cell survival after IR using clonogenic assay H shARv7 and/or oecircNHS effects on C4-2-IRR cells survival after IR using clonogenic assay. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the controls. N.S., not significant

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