Fig. 5
A Changes in ARv7 mRNA levels after different Que dose treatments. Control group is the 0 µM group. B Changes in ARv7 mRNA after 40 µM Que treatment at different times. Control group is the 0 H group. C Changes in AR and ARv7 protein levels in 22Rv1 and VCaP cells after different Que dose treatment. D Que and Enz effects on 22Rv1 cells survival after IR using clonogenic assay. E Que and Enz effects on C4-2-IRR cells survival after IR using clonogenic assay. F WB analysis of γ-H2AX levels in DMSO, Enz and Que groups after 4 Gy IR. G The average tumor volumes of the in vivo mouse model (each group N = 6) studies were recorded every 7 days and tumor volume (mm3) changes were graphed. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the controls. N.S., not significant