Fig. 3

ZNF498 directly interacts with p53 and represses p53 transcriptional activity by inhibiting p53 Ser46 phosphorylation. A, B Reciprocal co-IP of exogenous (A) and endogenous (B) ZNF498 and p53 in HepG2 cells. The indicated whole cell lysate and immunoprecipitates were analyzed by Western blotting. rIgG: rabbit IgG, mIgG: mouse IgG. C Direct interaction between ZNF498 and p53 as revealed by GST pulldown assays. D Map of the region of ZNF498 that interacts with p53. Cell lysates from HCT116 p53^−/− cells transfected with Myc-tagged deletion mutants of ZNF498 and Flag-tagged p53 were immunoprecipitated with an anti-Myc antibody before they were subjected to Western blotting. E Map of the region of p53 that interacts with ZNF498. Cell lysates from HCT116 p53^−/− cells transfected with Flag-tagged ZNF498 and Myc-tagged deletion mutants of p53 were immunoprecipitated with an anti-Myc antibody before they were subjected to Western blotting. FL: full length