Fig. 4

Co-administration of SAHA and veliparib enhanced PCa cell apoptosis. PCa cells LNCaP, C4–2 and PC-3 were treated with SAHA and veliparib alone or in combination at the indicated doses for 4 days (a LNCaP. b C4–2. c PC-3. d VCap, CWR22Rv1, DU145). Cells were stained with FITC-Annexin V antibody and counterstained with PI. The apoptotic cells were analyzed by flow cytometery. Representative dot plots of FITC-Annexin V/PI staining are shown. Graph shows mean apoptotic cells (Annexin-V+/PI+) ± SD. Experiments were performed in triplicate. Cell apoptosis was validated by testing the protein levels of cleaved PARP by western blotting (a-d). *p < 0.05; ** p < 0.01 (SAHA or Veliparib alone vs. co-treatment)