Fig. 1
FcγR expression profiling of human PBMCs cultured at low or high density for 48 h. a, Expression of FcγR on primary human monocytes (FSChiCD14+ cells) in low density (LD) or high density (HD) PBMC cultures determined using flow cytometry. Representative histograms above and quantified for 11 independent healthy donors below. b, Comparison of FcγR activating:inhibitory (A:I) ratio between LD and HD monocytes, (n = 11 per group). c, Quantification of FcγR and myeloid cell surface markers on monocytes in LD and HD PBMC cultures determined using flow cytometry and PE fluorescence quantitation beads, group means ± SD are shown, (n = 5 per group). d, Assessment of FcγRIIb expression by Western blot in LD and HD monocytes using CHO cells transfected with FcγRIIb1, and FcγRIIb2 isoforms as controls. e, Combined Western blot data of FcγRIIb expression normalized to HSC70 loading control (left) and fold change of FcγRIIb expression relative to HSC70 in LD and HD monocytes (right), (n = 16 per group). Each data point represents a unique healthy adult donor. Statistical significance between groups was assessed using a paired two-tailed Wilcoxon test (***p < 0.001, ****p < 0.0001 and ns = non-significant). Also see Additional file 1: Fig. S1