Fig. 4

Ectopic expression of circSCAP inhibits the proliferation of NSCLC cells in vivo. A Representative images of subcutaneous xenograft tumors of H1650 cells with circSCAP overexpressing or empty vector control (n = 5 for each group). B Representative images showed tumor morphology of xenograft in nude mice. C-D Ectopic expression of circSCAP suppressed the tumor growth (C) and decreased tumor weight (D) of xenograft tumors. E–F RT-PCR (E) and FISH (F) assay of the tumor tissues captured at the end point indicated the circSCAP was overexpressed in xenograft tumor. G Representative images of HE staining showed the pathological configuration of xenograft tumors (left: magnification, 200 × ; right: magnification, 400 ×). H-I Ki-67 (H) and PCNA (I) IHC staining showed the proliferation capacity of H1650 cells stably overexpressing circSCAP when compared with control cells in xenografts. * means P < 0.05. ** means P < 0.01, *** means P < 0.001