Fig. 2

PPP1R26 promotes cell proliferation in HCC cells. A Expression of PPP1R26 was evaluated by Western blot in the indicated cells stably expressing shRNA (left). Beta-actin was used as a loading control. Colony formation was performed with indicated cells (middle). Colony formation data were summarized from three independent experiments in triplicates and showed in histograms (right). B Cell viability was evaluated in the indicated cells stably expressing shRNA using MTS assays. C GFP-PPP1R26 or GFP was transfected in Huh7 and HepG2 cells. Expression of GFP-PPP1R26 was evaluated by Western blot (left). Beta-actin was used as a loading control. Colony formation assays were performed (middle). Colony formation data were summarized from three independent experiments in triplicates and showed in histograms (right). D MTS was used to evaluate cell proliferation in Huh7 and HepG2 cells expressing GFP-PPP1R26 or GFP, respectively. Data information: In (A-D), data are presented as mean ± SD. Statistical significance was assessed using two-tailed t-tests (C) or one-way ANOVA with post hoc analysis LSD test (A, B & D). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001