Fig. 3

PPP1R26 promotes cell migration and invasion in HCC cells. A Wound-healing experiments were done in Huh7 cells with PPP1R26 depletion (left). Line charts of relative wound area are shown (right). B Wound-healing experiments were done in HepG2 with PPP1R26 depletion (left). Line charts of relative wound area are shown (right). C Wound-healing experiments in Huh7 transfected with GFP or GFP-PPP1R26 (left). Line charts of relative wound area are shown (right). D Wound-healing experiments in HepG2 transfected with GFP or GFP-PPP1R26 (left). Line charts of relative wound area are shown (right). E Migration transwell experiments in Huh7 and HepG2 cells after knockdown of PPP1R26 or overexpression of GFP-PPP1R26 (left). Histogram analyses of relative migrated cell counts are shown (right). The migration cells were counted in five random fields under microscope. F Invasion transwell experiments in Huh7 and HepG2 cells after knockdown of PPP1R26 or overexpression of GFP-PPP1R26 (left). Histogram analyses of relative invasive cell counts are shown (right). The invasive cells were counted in five random fields under microscope. Data information: In (A-F), data are presented as mean ± SD. Statistical significance was assessed using two-tailed t-tests (C, D, E & F) or one-way ANOVA with post hoc analysis LSD test (A, E & F). **P < 0.01 and ****P < 0.0001