Fig. 8

PPP1R26 regulates the expression of EMT markers and PKM2 to promote HCC progression. A Proteins extracted from the xenografts were subjected to Western blotting probed with anti-PPP1R26, anti-GFP, anti-E-cadherin, anti-Vimentin, anti-Snail1 and anti-Twist2 antibodies. B-C Densitometry scanning analyses of the E-cadherin, Vimentin, Snail1 and Twist2 bands standardized to beta-actin are summarized and shown. D Western blot analysis of PPP1R26, E-cadherin, PTBP1, PKM2 and beta-actin expression in 16 paired HCC tissues. T denotes tumor tissues and P represents para-tumor non-tumorous tissues. E The correlation among PPP1R26 protein level with E-cadherin, PTBP1 and PKM2 protein level in the human HCC tissues was plotted by GraphPad. F A working model illustrating that PPP1R26 promotes HCC progression by simultaneously regulating glycolysis and EMT. Data information: In (B, C & E), data are presented as mean ± SD. Statistical significance was assessed using one-way ANOVA with post hoc analysis LSD test (B & C) or two-tailed t-tests (E). *P < 0.05, ***P < 0.001 and ****P < 0.0001