Fig. 6
From: Inhibition of the mitochondrial protein Opa1 curtails breast cancer growth
OPA1 ablation increases miR-148a, miR-148b and miR-152 expression level. a Volcano plot of differentially expressed miRNAs in MDA-MB-231 transfected for 72 h with siRNA against OPA1 or a non-relevant sequence (UNREL). Blue dots correspond to miRNAs significantly up-regulated in Opa1-ablated MDA-MB-231. n = 4 independent experiments. b miR-148/152 family. Mature human and mouse sequences for miR-148a, miR-148b and miR-152. Seed sequences are colored in red. c 2-Δct of mir148a levels determined by qRT-PCR in MDA-MB-231, T47D, MCF7, HS578T transfected as indicated for 72 h. n = 4 independent experiments. **: p < 0.001. d 2-Δct of mir148b levels determined by qRT-PCR in MDA-MB-231, T47D, MCF7, HS578T transfected as indicated for 72 h. n = 4 independent experiments. **: p < 0.001. e 2-Δct of mir152 levels determined by qRT-PCR in MDA-MB-231, T47D, MCF7, HS578T transfected as indicated for 72 h. n = 4 independent experiments. **: p < 0.001. f 2-Δct of mir148a levels determined by qRT-PCR in MDA-MB-231, T47D, MCF7, HS578T treated as indicated for 8 h. n = 4 independent experiments. **: p < 0.001. g 2-Δct of mir148b levels determined by qRT-PCR in MDA-MB-231, T47D, MCF7, HS578T treated as indicated for 8 h. n = 4 independent experiments. **: p < 0.001. h 2-Δct of mir152 levels determined by qRT-PCR in MDA-MB-231, T47D, MCF7, HS578T treated as indicated for 8 h. n = 4 independent experiments. **: p < 0.001. i 2-Δct of mir148a levels determined by qRT-PCR in MDA-MB-231 with the indicated genotype. n = 4 independent experiments. ***: p < 0.0001 #: p < 0.0001. j 2-Δct of mir148b levels determined by qRT-PCR in MDA-MB-231 with the indicated genotype. n = 4 independent experiments. ***: p < 0.0001 #: p < 0.0001. k 2-Δct of mir152 levels determined by qRT-PCR in MDA-MB-231 with the indicated genotype. n = 4 independent experiments. ***: p < 0.0001 #: p < 0.0001