Fig. 7

OPA1 ablation inhibits breast cancer cells hallmarks by increasing miRNA 148a, 148b and 152 expression. a Heat map of the major pathways dysregulated by the change of the miRNA-148a, 148b and 152. Data are resorted from http://snf-515788.vm.okeanos.grnet.gr/#mirnas=hsa-miR-148a-3p;hsa-miR-148b-3p;hsa-miR-152-3p&methods=microT-CDS;microT-CDS;microT-CDS&selection=2. b Quantification of cell migration after 6 h of MDA-MB-231 transfected as indicated for 24 h. n = 19 independent experiments. **: p < 0.001. P-: miRNA mimic. c Quantification of proliferation of MDA-MB-231 transfected as indicated for 24 h determined by BrdU incorporation. n = 4 independent experiments. **: p < 0.001. P-: miRNA mimic. d Quantification of cell adhesion on fibronectin of MDA-MB-231 transfected as indicated for 24 h. n = 4 independent experiments. **: p < 0.001. P-: miRNA mimic. e Equal amounts of protein from breast cancer cells MDA-MB-231 transfected 24 h as indicated were separated by SDS-PAGE and immunoblotted with the indicated antibodies. Numbers above each panel represent densitometric quantification. P-: miRNA mimic. f-h Quantification of cell migration (f), proliferation (g) and adhesion (i) of MDA-MB-231 (transfected with the indicated siRNA for 72 h and indicated anti-miRNA for 24 h. n = 4 independent experiments. *: p < 0.05, ***: p < 0.0001 and #: p < 0.05. A-: Anti miRNA. i-k Quantification of migration (i), proliferation (j) and adhesion (k) of T47D transfected with the indicated siRNA for 72 h and indicated anti-miRNA for 24 h and determined by BrdU incorporation. n = 4 independent experiments. *: p < 0.05, ***: p < 0.0001 and #: p < 0.05. A-: Anti miRNA. l-n Quantification of cell migration (l), proliferation (m) and adhesion (n) of MCF7 transfected with the indicated siRNA for 72 h and indicated anti-miRNA for 24 h. n = 4 independent experiments. *: p < 0.05, ***: p < 0.0001 and #: p < 0.05. A-: Anti miRNA. o-q Quantification of cell migration (o), proliferation (p) and adhesion (q) of HS578T transfected with the indicated siRNA for 72h and indicated anti-miRNA for 24h. n=4 independent experiments. *: p < 0.05, ***: p < 0.0001 and #: p < 0.05. A-: Anti miRNA.  r Quantification of cell migration after 6 h of MDA-MB-231 with the indicated genotype and transfected as indicated for 24 h. n = 4 independent experiments. *: p < 0.05, ***: p < 0.0001 and #: p < 0.05. A-: Anti miRNA. s Quantification of proliferation of MDA-MB-231 with the indicated genotype and transfected as indicated for 24 h and determined by BrdU incorporation n = 4 independent experiments. *: p < 0.05, ***: p < 0.0001 and #: p < 0.05. A-: Anti miRNA. t Quantification of cell adhesion on fibronectin of MDA-MB-231 with the indicated genotype and transfected as indicated for 24 h. n = 4 independent experiments. *: p < 0.05, ***: p < 0.0001 and #: p < 0.05. A-: Anti miRNA