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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: miR-3189-targeted GLUT3 repression by HDAC2 knockdown inhibits glioblastoma tumorigenesis through regulating glucose metabolism and proliferation

Fig. 3

miR-3189 suppresses tumorigenesis of in vivo mouse GBM. A qPCR analysis of miR-3189 expression in GBM cells transfected with miR-3189 mimics. B Cell viability was measured in miR-3189-transfected GBM cells by MTT assay. C PARP and GLUT3 expression were measured in GLUT3 siRNA- or miR-3189-transfected GBM cells by western blot. D Cell death markers were analyzed by western blot with indicated antibodies in miR-3189 mimic-transfected GBM cells. E Schematic outline of in vivo experimental procedure. F Mouse bodyweight of in vivo mouse GBM models. G PET-CT imaging in orthotopic mouse GBM models by miR-3189 transfection. H SUV image analysis (mean area) in miR-3189-expressing mouse GBM models. Graphic representation of SUV image analysis (mean area) rate of each group. I Tumorigenesis in orthotopic mouse GBM models by miR-3189 transfection. H&E staining of brain sections of mice. J GLUT3 expression in mouse GBM tissues. K IHC analysis of GLUT3 and Bax in mouse GBM tissues transfected miR-3189. Representative images of mouse GBM tissue. Scale bar: 100 μm. L mRNA expression of miR-3189, GLUT3, Bax, and Apaf-1 in mouse GBM tissues transfected miR-3189. All data are expressed as the mean ± SD from three independent experiments, each performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001

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