Fig. 3

Activation of antigen specific TILs and B cells in a spontaneously metastatic model of TNBC. A In vitro cytotoxicity of splenocytes towards 4T1 cells. B Generation of antigen (ASPH) specific CD4⁺ and CD8⁺ T cells as demonstrated by upregulation of IFN-γ. C-D Number of CD3⁺ TILs in TNBC primary tumors and E-F pulmonary metastases. G ASPH specific antibody titers in serum derived from different groups. H-J Number of CD62L⁺/CD8⁺ effector CTLs in primary tumors and pulmonary metastases. K-M Number of CD44⁺ memory CTLs in primary tumors and pulmonary metastases. ^*, p < 0.05; ^**, p < 0.01; ^***, p < 0.005; ^****, p < 0.001