Fig. 5

ZMAT1 functions in a p53-dependent manner. A Gene set enrichment analysis (GSEA) of RNA sequencing on SW1990/Vector and SW1990/ZMAT1-OV cells groups. B Heatmap of RNA sequencing showed differential expression levels of key nodes in p53-assicoated cell cycle and apoptosis pathways. C-D RT-qPCR and western blot were used to detect the p53 levels in ZMAT1 over-expressed (C) and knockdown cells (D). E Double-label immunofluorescence staining for the intracellular localization of ZMAT1 and p53 in SW1990 cells. F luciferase activity assays were performed on 293 T cells with co-transfection of pGL3-p53 with 0.01 μg, 0.05 μg, 0.1 μg, 0.5 μg and 1 μg of vector encoding ZMAT1. G SW1990/ZMAT1-OV cells were treated with Pifithrin-α for 24 h and the protein levels of ZMAT1, p53, p21, and BAD were analyzed by immunoblotting with the indicated antibodies. H CCK-8 assays were performed on SW1990/ZMAT1-OV cells after treating with Pifithrin-α for 24 h. I Colony formation assays were performed on SW1990/ZMAT1-OV cells after treating with Pifithrin-α for 24 h. All * P-value < 0.05, ** P-value < 0.01, *** P-value < 0.001. P-values were assessed using two-tailed t-tests and ANOVA followed by Dunnett’s tests for multiple comparison in B, C, D, E, G, J and K. All figures represent mean ± SD from three independent experiments