Skip to main content
Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Correction to: Elafin promotes tumour metastasis and attenuates the anti-metastatic effects of erlotinib via binding to EGFR in hepatocellular carcinoma

Fig. 3

Overexpression of Elafin promotes EMT and metastasis of HCC cells in vitro and in vivo. a The efficiency of Elafin overexpression were measured by real-time PCR (top) and Western blotting (bottom) assays. *** P < 0.001. b Overexpression of Elafin enhanced migration and invasion of MHCC-97H, Huh7 and Hep3B cells. Scale bar, 100 μm. Statistical results are presented as mean ± SD (from triplicates), and significance is determined by Student t test (*** P < 0.001). c Overexpression of Elafin enhanced the scratch wound-healing ability of MHCC-97H, Hep3B and Huh7 cells. Scale bar, 100 μm. Statistical results at 48 h are presented as mean ± SD (from triplicates) (** P < 0.01; *** P < 0.001). d and e Concentrated Elafin-overexpression condition medium (Elafin CM) and commercial recombinant Elafin (rElafin, 10 μg/ml) promoted migration and invasion of wild-type HCC cells. Scale bar, 100 μm. Statistical results are presented as mean ± SD (from triplicates) (* P < 0.05; ** P < 0.01;*** P < 0.001). f The effects of concentrated Elafin-knockdown conditioned medium on HCC cells. Scale bar, 100 μm. Statistical results are presented as mean ± SD (from triplicates) (* P < 0.05; ** P < 0.01). g Representative immunofluorescence images illustrating cytoskeleton of control and Elafin overexpressing cells. Scale bar, 20 μm. h Representative immunofluorescence images of EMT markers expression in Elafin overexpressing cells. Scale bar, 50 μm. i Expression of EMT markers mediated by Elafin overexpression are shown by western boltting. j Overexpression of Elafin significantly enhanced lung metastasis in nude mice model established by injection of indicated cells through the tail vein. Representative HE staining images are shown. Scale bar, 100 μm. k Lung metastasis nodules from the nude mice model are analyzed. *** P < 0.001

Back to article page