Fig. 5

STC1 is directly regulated by FOXC2 to activate the PI3K signaling pathway, participating in lipid metabolism in vitro. A Ten transcription factor families that may be related to STC1 were identified by RNA sequencing. B-C Stable knockdown of FOXC2 expression in Skov3-ip1 and Hey cells was confirmed by WB and real‐time PCR assays. D ChIP results of the binding of FOXC2 to the promoter of STC1. E The luciferase reporter assay showed that co-transfection of the STC1 MT plasmid with the FOXC2 plasmid significantly decreased luciferase activity. F IF analysis showed the co-localization of FOXC2 and STC1 in Skov3-ip1 and Hey cell lines. G PCA of data from untargeted relative quantitative lipidomic analysis. H Volcano plot of individual lipid species significantly up- or down- regulated in the NC group versus the FOXC2-sh1 and FOXC2-sh2 groups in Hey cell lines (lipids with a P value < 0.05 and a fold change (FC) > 1.5 or < 0.67 were indicated in blue or red, respectively). I Correlation analysis of the 12 lipid classes. J Representative WB results of ITGB6 and proteins in the PI3K signaling pathway in Skov3-ip1 and Hey cell lines. (Data are shown as the mean ± SD values. Significance was calculated using Student’s t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.)