Fig. 1

OVOL2 is down-regulated in ATC and its re-expression drives MET transcriptional program. A Correlation of OVOL2 expression with Epithelial and Mesenchymal gene signature from the THCA-TCGA database. B OVOL2 expression levels in different cell lines derived from immortalized thyrocytes, primary and metastatic Differentiated Thyroid Carcinoma (DTC) and Anaplastic Thyroid Carcinoma (ATC). C, B Assessment of OVOL2-HA expression by qRT-PCR C and IF D in 8505c and 8305c cells. IF were performed 48 h after induction. E–G Evaluation of a panel of epithelial and mesenchymal markers upon OVOL2 induction in ATC cell lines by qRT-PCR (normalized on EV cells) E, H flow cytometry Staining F and western blotting G. Histograms represent means and SD of independent biological replicates, *p < 0,05, **p < 0,005