Fig. 5

OVOL2 expression impairs pathways related to cell cycle and cytoskeleton dynamics. A Heatmap depicting hierarchical clustering based on the 5762 differentially expressed genes, whose read counts are Z-score normalized. Unsupervised hierarchical clustering was performed between 8505c-EV and 8505c-OVOL2 biological replicates. Color intensity for each gene shows Z-score values ranging from red for up-regulation and green for down-regulation. B Volcano plot displaying significantly de-regulated genes (adjusted P value and log2 FC) between 8505c EV and OVOL2-expressing cells. C, D GO and Reactome enrichment analysis on OVOL2-deregulated genes from RNAseq analysis. Pathways majorly affected by OVOL2 induction are displayed, ranked for P value in each group. Total number of genes enriched in each pathway are reported on the bars. E RNA-seq validation of a panel of the most down-regulated genes by qRT-PCR, on 8505c and 8305c 24 h upon OVOL2 induction, normalized on EV cells. All genes are significantly down regulated (p < 0,05) unless where the “ns” label is present (p > 0,05). Histogram represents means and SD of two independent biological replicates. F Protein–protein interaction network of 222 OVOL2 specific targets