Fig. 6

OVOL2 effects depend on RhoU repression. A ChIP assay with anti-HA antibody or control normal IgG, in 8505c cells, presented as % of input. An unrelated DNA region was used as negative control while N-cadherin promoter region was used as positive control. Significance is calculated comparing each target to the negative control region. B-E Proliferation B, C and migration ability D, E of 8505c and 8305c respectively, after transfection with siRNA specific for RhoJ, RhoU or the combination of both, compared to control siRNA. Graphs represent means and SD of two independent biological replicates *p < 0,05, **p < 0,005. F Schematic representation of the effect of OVOL2 expression on different cellular process in ATC cells (Created with BioRender.com)