Fig. 3

Analysis of hOA-DN30 cancer cell growth inhibition in vitro. a Analysis of cell viability by quantitation of metabolically active cells in a panel of cancer cell lines treated with increasing concentrations of hOA-DN30 for 72 h. Black: cancer cells carrying diploid or low amplified MET gene; Red: cancer cells carrying high MET gene amplification. Gcn: gene copy number. For each cell line, viable cells are reported as the percentage of untreated cells. Each point is the mean of triplicate values; bars represent SD. b Analysis of cell proliferation by EdU incorporation. Cytofluorimeter analysis of the indicated MET-amplified cells treated with 1 μM hOA-DN30 for 48 h. Ctrl: untreated cells. The percentage of EdU-positive cells is indicated in the plots. FS: Forward Scatter. c Analysis of cell death by incorporation of CellTox Green Dye. MET-amplified cells treated with increasing concentrations of hOA-DN30 for 72 h. Graphs report the fold increase of cytotoxicity versus untreated cells. Each point is the mean of triplicate values; bars represent SD