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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Mechanical stress shapes the cancer cell response to neddylation inhibition

Fig. 1

Neddylation inhibition induces distinct phenotypes in PCa cells. A, B Effect of 100 nM MLN on PCa colony growth in soft agar (“steady”). In the “pulse” regimen, the cells were pretreated with 100 nM MLN for 1 h before seeding (see also Supplementary Figure S1A). The histograms show the colony size distribution of control (black)- and MLN (red)-treated cells. The numbers indicate the total area occupied by the colonies of the given size. Scale bar = 1 mm. C, D Wound healing assay with LNCaP (C) and PC3 (D) cells (mean ± S.D., n = 5 for LNCaP, n = 6 for PC3, ***-p < 0.001). 100 nM MLN was added just before monolayer scratching. Scale bar = 500 µm (E, F) Effect of 100 nM MLN on LNCaP (E) and PC3 (F) tumoroid growth over 10 days. Scale bar = 150 µm. In all experiments with MLNs, DMSO was used as a vehicle control

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