Skip to main content
Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Surfaceome analyses uncover CD98hc as an antibody drug-conjugate target in triple negative breast cancer

Fig. 1

Identification of surface proteins differentially expressed in TNBC. A Schematic representation of the genomic and proteomic approaches. For the genomic approach, microarray data from normal and tumoral triple negative breast cancer of patients obtained by us (SUH) or deposited in databases (HBS and TBC) were used. The number of up-regulated cell surface proteins found using each dataset is shown. The proteomic approach was based on cell surface biotinylation and plasma membrane enrichment to detect surface proteins from MDA-MB231, BT549 and HS578T cells. Upon Orbitrap identification, plasma membrane proteins were selected using the Surfaceome database. B List of possible protein targets (score ≥5) ranked from highest to lowest score. The scoring criteria (one point per analysis) are described in the main text of this paper. The maximum score of 9, would be given to a protein identified in the three cell lines in the two proteomic methods and also identified in the three gene expression arrays. C Levels of expression of LAT1, CD98hc and GLUT1 in a panel of TNBC cell lines. Cell extracts of different TNBC cell lines were used to identified LAT1, CD98hc and GLUT1 by Western blot. Calnexin was used as a loading control. D Quantitation of expression of CD98hc and LAT1 of the experiment shown in (C). The graph represents the expression values of CD98hc and LAT1 for each cell line. Quantitation of CD98hc and LAT1 was made as described in the experimental procedures section. Pearson’s correlation coefficient and the p value are shown. E Co-immunoprecipitation studies of CD98hc and LAT1. One mg of HCC3153 extracts were immunoprecipitated with the anti-CD98hc antibody and the immunocomplexes were analyzed by Western with the anti-LAT1 antibody. Mouse IgG was used as a control. F Expression of CD98hc and LAT1 in tumoral samples of patients with TNBC. The tumours were homogenized and lysed. CD98hc and LAT1 were analyzed by Western blot. β-actin was used as a loading control. G Quantitation of expression of CD98hc and LAT1 of the experiment performed in (F). The graph represents the expression values of CD98hc and LAT1 for each tumor sample. Pearson’s correlation coefficient and the p value are shown

Back to article page