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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: PPFIA4 promotes castration-resistant prostate cancer by enhancing mitochondrial metabolism through MTHFD2

Fig. 1

The expression of PPFIA4 is upregulated in CRPC. A-B. The mRNA and protein levels of PPFIA4 in LNCaP cells determined by qRT-PCR and western blotting. LNCaP cells were treated with 0.1, 1, and 10 nM R1881 for 24 hours (A) or treated with 1 nM R1881 at the indicated time points (B). Western blotting was performed with the indicated antibodies. GAPDH was used as a loading control. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 based on the Student’s t-test. h, hours. C. The mRNA and protein levels of PPFIA4 in LNCaP cells with prolonged androgen-deprived treatment (1, 2, and 3 months) determined by qRT-PCR and western blotting. Western blotting was performed with the indicated antibodies. GAPDH was used as a loading control. **p < 0.01, ****p < 0.0001 based on the Student’s t-test. CSS, charcoal-stripped serum. m, months. D-G. PPFIA4 expression in metastatic CRPC (mCRPC) tissues compared to primary localized PCa samples and in PCa patients with different Gleason scores in public datasets (GSE68882, GSE35988, GSE6919 and GSE21034). *p < 0.05, **p < 0.01, ****p < 0.0001. H. Kaplan–Meier survival analysis of PCa cases from GSE46602 (p = 0.034, Log-rank test) and GEPIA prostate cohort (p = 0.017, Log-rank test). m, months. I. Representative IHC images of PPFIA4 in localized PCa and mCRPC tissues. Magnified images from the regions marked by rectangles were showed in the bottom. J. Percentages of different PPFIA4 expression (out of 100%) distributed in 157 PCa cases in Qilu Hospital with primary localized PCa or CRPC

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