Fig. 4

Ex vivo effects of PepA and ephrinA1-Fc treatments on GSCs. A Ex vivo treatment of GSCs with exogenous PepA hinders invasiveness of either I- (top) or D-GSCs (bottom) regardless of subtype. Bars, 50um Quantification is shown in B as mean ± SEM. ***P < 0.001, *P < 0.05, ns, not significant, one-way Student’s t-test; n = 3. C. Exposure of I- (left) and D-GSCs (right) to PepA (green line), ephrinA1-Fc (red line) or a combination of both (purple line) lessens steady growth of both GSCs to nearly half than in control GSCs, showing a clear additive effect of the two molecules. Data are mean ± SD. ***P < 0.001 I-GSCs vs. D-GSCs, hierarchical linear model for repeated measurements; n = 3