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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Growth factor independence underpins a paroxysmal, aggressive Wnt5aHigh/EphA2Low phenotype in glioblastoma stem cells, conducive to experimental combinatorial therapy

Fig. 5

A combinatorial PepA/ephrinA1-Fc-manipulation approach impairs tumorigenicity and invasiveness of GSCs cells. A. Quantitative analysis of luc-GSCs signal showing that both PepA and ephrinA1-Fc hinder I-GSCs-derived tumors’ growth (left) and, to a lesser extent, of their D-GSCs counterpart (right) and that the combined use of the two molecules has an additive effect. Data are mean ± SEM. ***P < 0.001, *P < 0.05, Dunnett’s multiple comparison test; n = 5 mice. B. Imaging of luciferase-tagged I- and D-GSCs injected into the brain of Scid/bg mice showing that after 23 and 34 days, respectively, untreated mice develop larger and spreaded tumors than PepA and ephrinA1-Fc-infused mice. Tumor growth is markedly inhibited with the combination of both molecules. C. Brain sections confirming that tumors from mice carrying I-GSCs or D-GSCs cells and infused with vehicle proliferated and spread through the brain parenchyma more than those infused either with PepA or ephrinA1-Fc, being the combination of both even more efficacious in reducing cell proliferation and invasiveness. Bar, 1 mm. D. Survival of mice harboring I-GSCs (left) and D-GSCs-tumors (right) is significantly enhanced when infused with PepA (blue bar) and ephrinA1-Fc (red bar) alone, or even more with the combination of the two molecules (green bar). A league table of comparison by Log-rank is shown; n = 5 mice

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