Fig. 5

Combined Deficiency of FEN1 and DNA-PKcs Triggers DNA Damage Accumulation and Genomic Instability. a, Comet analysis in M059K cells treated with sc-13 or/and NU-7441 upon HU exposure. Representative images are shown. b Immunofluorescence labeling to detect foci of γ-H2AX and 53BP1 in cells treated with sc-13 or/and NU-7441. c, d Images of mitotic spreads and micronucleo forms M059K cells treated with sc-13 or/and NU-7441 following exposure to HU. Scale bar, 5 µm. e Bar chart illustrating DNA damage accumulation of comet assay. f, g Bar chart illustrating elevated level of γ-H2AX and 53BP1 in M059K cells treated with sc-13 or/and NU-7441 upon HU exposure from three independent replicates. Data are mean ± s.d. h, i Bar chart illustrating increased level of tail moment in M059K cells treated with sc-13 or/and NU-7441 upon HU exposure from three independent replicates. Data are mean ± s.d. A two-sided unpaired t test was used to calculate P-values. NS: not significant: P > 0.05