Fig. 6From: Flap endonuclease 1 and DNA-PKcs synergistically participate in stabilizing replication fork to encounter replication stress in glioma cellsCombined Loss of FEN1 and DNA-PKcs Exacerbates Reduced Cellular Progression and Cell Viability. a Ethynyldeoxyuridine (EdU) detection is carried out by FACS assay. Images of different stage of cells are shown. b Quantification are represented as means ± s.d from at least three independent experiments. c Colony formation assay of U251 cells treated with sc-13 or/and NU-7441. Representative images are shown. d Quantification are represented as means ± s.d from at least three independent experiments. e Cell viability analysis are generated from CCK8 test. Data are represented as means ± s.d from at least three independent experiments. f, g Images and quantification of invasion and migration experiments of U251 cells treated with sc-13 or/and NU-7441 are shown. h Quantification are represented as means ± s.d from at least three independent experiments. i Clonogenic assay was performed with indicated dose of sc-13 for ten days. Representative pictures are shown. j, k Surviving fraction was calculated. l Viability assay in multiple cell lines treated with sc-13 and VX-984. A two-sided unpaired t test was used to calculate P-values. NS: not significant: P > 0.05Back to article page