Fig. 1From: HIF1α-SP1 interaction disrupts the circ-0001875/miR-31-5p/SP1 regulatory loop under a hypoxic microenvironment and promotes non-small cell lung cancer progressionExpression and characterization of circ-0001875 in NSCLC cells and tissues. a Volcano plots showing the differentially expressed circRNAs in NSCLC tissue relative to matched normal tissue. b The top eight dysregulated circRNAs are listed. c RT-qPCR analysis of the eight most dysregulated circRNAs in A549, PC9, H838, H1299 and 95D cells compared with expression in BEAS-2B cells. d Relative expression levels of circ-0001875 in BEAS-2B cells and NSCLC cell lines were determined by RT-qPCR. e–f Relative expression of circ-0001875 in NSCLC tissues and adjacent nontumor tissues was detected by RT-qPCR (n = 72). g A schematic of the genomic location and back splicing of circ-0001875 with the splicing site validated by Sanger sequencing. h–i Circ-0001875 and FAM120A mRNA expression were detected in NSCLC cells after Actinomycin D and RNase R treatment. j FISH assay showed that circ-0001875 (FITC-labeled probe) was abundant in the cytoplasm, DAPI was used to stain cell nuclei. Images are shown at 600 × magnification. Scale bar = 20 μm. Data are shown as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001Back to article page