Fig. 6

Circ_0001875 regulates malignant phenotypes in lung cancer cells via the miR-31-5p/SP1 axis. a–c Relative expression of SP1 mRNA and protein in cells transfected with three short hairpin RNAs (si-SP1) and an overexpression vector of SP1 (pcDNA SP1). d–j CCK8, colony formation, wound healing assay and Transwell assays in the four experimental groups shown (si-NC + vector, si-NC + pcDNA SP1, si-circ-0001875 + vector, si-circ-0001875 + pcDNA SP1). Images were obtained at 100 × magnification. Scale bar = 100 μm. Data are shown as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001