Fig. 7

Circ_0001875 via the miR-31-5p/SP1 axis promotes EMT through a TGFβ/Smad2 signal pathway. a Western blot analysis of p-Smad2, Smad2 and EMT-associated molecules in cells with circ-0001875 knockdown and overexpression treatment. b–c Western blot analysis of p-Smad2, Smad2 and EMT-associated molecules in cells treated as indicated. d Western blot analysis of p-Smad2, Smad2, EMT-associated molecules and SP1 were analyzed in cells transfected with the indicated vectors and treated with TGFβ/Smad pathway inhibitor (Galunisertib). e Immunofluorescent staining of F-actin to detect filopodia in the circ-0001875 overexpression group and vector group. Images were obtained at 600 × magnification. Scale bar = 40 μm. Data are shown as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001